Phosphoinositides. 3. Enzymic hydrolysis of inositol-containing phospholipids.
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چکیده
منابع مشابه
Myo-inositol phosphates obtained by alkaline hydrolysis of beef brain phosphoinositide.
It is now apparent that the nutritional requirement for myoinositol in many cells is related to its participation as a building unit of myo-inositol-containing phospholipids (phosphoinositides). This explains why Eastcott (1)) in her classic work, found that all the myo-inositol taken up from the medium by growing yeast could be recovered by acid hydrolysis of the cells. The role of phosphoinos...
متن کاملGuanine nucleotides stimulate production of inositol trisphosphate in rat cortical membranes.
The guanine nucleotides guanosine 5'[beta, gamma-imido]triphosphate (Gpp[NH]p), guanosine 5'-[gamma-thio]-triphosphate (GTP gamma S), GMP, GDP and GTP stimulated the hydrolysis of inositol phospholipids by a phosphodiesterase in rat cerebral cortical membranes. Addition of 100 microM-Gpp[NH]p to prelabelled membranes caused a rapid accumulation of [3H )inositol phosphates (less than 30 s) for u...
متن کاملInositol phospholipids and cell regulation.
The increased turnover of phosphatidylinositol (PtdIns) in response to activation of cell-surface receptors was discovered by Hokin & Hokin (1953). The structures of the so-called polyphosphoinositides, phosphatidylinositol 4-phosphate (Ptdlns4P) and phosphatidylinositol 4,5-bisphosphate [PtdIns(4,5)P2], were not elucidated until the next decade, but their metabolic importance was suggested by ...
متن کاملInositol trisphosphate and diacylglycerol as second messengers.
Hormones and neurotransmitters that use calcium as a second messenger specifically hydrolyse membrane phosphoinositides. This perturbation of membrane phospholipids may represent a fundamental transducing mechanism that initiates a signal cascade resulting in the mobilization of calcium, the activation of protein kinase C, the release of arachidonic acid (for the synthesis of prostaglandins, th...
متن کاملDirect labelling of hormone-sensitive phosphoinositides by a plasma-membrane-associated PtdIns synthase in turkey erythrocytes.
We have previously characterized phosphatidylinositol (PtdIns) synthase and PtdIns/myo-inositol-exchange enzyme activities in ghost membranes prepared by hypotonic lysis of turkey erythrocytes [McPhee, Lowe, Vaziri and Downes (1991) Biochem. J. 275, 187-192]. Here we show that PtdIns synthase activity is relatively enriched in plasma-membrane preparations of turkey erythrocytes and that inosito...
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ورودعنوان ژورنال:
- The Biochemical journal
دوره 79 شماره
صفحات -
تاریخ انتشار 1961